Journal: Molecular Cell

Article Title: A cooperative PNPase-Hfq-RNA carrier complex facilitates bacterial riboregulation

doi: 10.1016/j.molcel.2021.05.032

Figure Lengend Snippet:

Article Snippet: 3′,5′-cyclic AMP.Na (cAMP) , Chem-Impex Int’l Inc. , Cat#00008.

Techniques: Recombinant, Software

Journal: Bioactive Materials

Article Title: Surface decoration of development-inspired synthetic N-cadherin motif via Ac-BP promotes osseointegration of metal implants

doi: 10.1016/j.bioactmat.2020.11.002

Figure Lengend Snippet: (A) Diagram illustrating biomimetic peptide/acryloyl-bisphosphonate (Ac-BP) conjugation and hMSCs seeding on 2D titanium substrates, (B) osseointegration in the site of the implantation with the biomimetic peptide/Ac-BP conjugated titanium screws in rabbit tibiae/femurs ( in vivo ). (C) And the reaction of biomimetic peptide conjugation to TiO 2 substrates with Ac-BP.

Article Snippet: Human MSCs seeded on the 2D biomimetic peptide/Ac-BP functionalized titanium substrates were fixed in 4% paraform for 10 min, rinsed in phosphate-buffered saline (PBS) for at least three times, and permeabilized with 0.25% TritonX-100 in PBS for 30 min at room temperature.

Techniques: Conjugation Assay, In Vivo

Journal: Bioactive Materials

Article Title: Surface decoration of development-inspired synthetic N-cadherin motif via Ac-BP promotes osseointegration of metal implants

doi: 10.1016/j.bioactmat.2020.11.002

Figure Lengend Snippet: (A) Gene expression of osteogenic markers (Osterix and Osteomodulin) in the hMSCs seeded on the biomimetic peptide/Ac-BP conjugated TiO 2 surface after osteogenic differentiation for 4 days and 12 days. (*p value < 0.05, **p value < 0.01 vs. Cad + RGD, n = 4). (B) RUNX2 immunostaining of the hMSCs seeded on 2D titanium substrates (Control), and the substrates conjugated with Ac-BP and N-cadherin/RGD mimetic peptide (Cad + RGD), Scrambled sequence N-cadherin/RGD mimetic peptide (Scram + RGD), or RGD mimetic peptide (RGD) after 4 days of osteogenic culture. (Scale bar = 250 μm) (C) The atomic force microscope (AFM) images of group Cad + RGD, Scram + RGD, RGD and control. The untreated titanium substrates were measured as control (The test area of AFM analysis is 10 μm*10 μm as shown in figure). (D) X-ray photoelectron spectroscopy (XPS) spectra of corresponding groups (a–d) to the AFM scanning result, showing P–O peaks after the reaction with biomimetic peptide/acryloyl-bisphosphonate and washed with distilled water. While images (e–h) also show the grouped peaks measured 12 days after the surface coating reaction, which demonstrated the successful reaction of Ac-BP and biomimetic peptide on the titanium surface by forming titanium-oxygen-phosphorus group (Ti–P–O), and the coating would last after 12 days cultivation (n = 3).

Article Snippet: Human MSCs seeded on the 2D biomimetic peptide/Ac-BP functionalized titanium substrates were fixed in 4% paraform for 10 min, rinsed in phosphate-buffered saline (PBS) for at least three times, and permeabilized with 0.25% TritonX-100 in PBS for 30 min at room temperature.

Techniques: Gene Expression, Immunostaining, Control, Sequencing, Microscopy, Spectroscopy